منابع مشابه
Genome-Wide Mapping of DNA Strand Breaks
Determination of cellular DNA damage has so far been limited to global assessment of genome integrity whereas nucleotide-level mapping has been restricted to specific loci by the use of specific primers. Therefore, only limited DNA sequences can be studied and novel regions of genomic instability can hardly be discovered. Using a well-characterized yeast model, we describe a straightforward str...
متن کاملMapping Meiotic Single-Strand DNA Reveals a New Landscape of DNA Double-Strand Breaks in Saccharomyces cerevisiae
DNA double-strand breaks (DSBs), which are formed by the Spo11 protein, initiate meiotic recombination. Previous DSB-mapping studies have used rad50S or sae2Delta mutants, which are defective in break processing, to accumulate Spo11-linked DSBs, and report large (> or = 50 kb) "DSB-hot" regions that are separated by "DSB-cold" domains of similar size. Substantial recombination occurs in some DS...
متن کاملCorrection: Mapping Meiotic Single-Strand DNA Reveals a New Landscape of DNA Double-Strand Breaks in Saccharomyces cerevisiae
(2007) Mapping meiotic single-strand dna reveals a new landscape of DNA double-strand breaks in Saccharomyces cerevisiae. PLoS Biol 5(12): e324. Two figure legends refer to the wrong strain of yeast. In Figure 2, the second sentence of (B) should say: Spo11 enrichment ratios for rad50S (MJL2404) were determined from input samples and immunoprecipitates (ChIP). In Figure 3, the first sentence sh...
متن کاملDNA Double-Strand Breaks
The activation-induced cytidine deaminase (AID) is required for somatic hypermutation (SHM) and class-switch recombination (CSR) of immunoglobulin (Ig) genes, both of which are associated with DNA double-strand breaks (DSBs). As AID is capable of deaminating deoxy-cytidine (dC) to deoxy-uracil (dU), it might induce nicks (single strand DNA breaks) and also DNA DSBs via a U-DNA glycosylase-media...
متن کاملSingle nucleotide-level mapping of DNA double-strand breaks in human HEK293T cells
Constitutional biological processes involve the generation of DNA double-strand breaks (DSBs). The production of such breaks and their subsequent resolution are also highly relevant to neurodegenerative diseases and cancer, in which extensive DNA fragmentation has been described Stephens et al. (2011), Blondet et al. (2001). Tchurikov et al. Tchurikov et al. (2011, 2013) have reported previousl...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2020
ISSN: 1548-7091,1548-7105
DOI: 10.1038/s41592-020-0876-y